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KMID : 0613820040140040657
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Journal of Life Science
2004 Volume.14 No. 4 p.657 ~ p.663
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Expression of Cinnamic Acid 4-Hydroxylase Chimeric Gene Fused with Sesquiterpene Cyclase Promoter from Hot Pepper in Tobacco
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Lee Gyung-Min
Yoon Yong-Hwi
Kim Kil-Woong
Lee In-Jung
Shin Dong-Hyun
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Abstract
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Tobacco transformants harboring cinnamic acid 4-hydroxylase gene (C4H) fused with susquiterpene cyclase promoter was developed in order to regulate biosynthesis of phenolic compounds by the expression of the introduced gene. Twenty transformants for each specific promoter were used to analyze the incorporation of the chimeric genes by PCR and Southern blot analysis. PCR products of NPTⅡ (neomycin phosphotransferase) gene (553bp) were detected in the transgenic tobacco plants. The incorporation of the chimeric gene was confirmed in the Southern blot analysis. C4H activity in the transgenic plants was elevated by UV-irradiation and its level was higher compared to that of control plants.
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KEYWORD
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cinnamic acid 4-hydroxylase, cinnamic acid 4-hydroxylase activity, susquiterpene cyclase promoter, transgenic tobacco
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